2 edition of Pollen-specific gene expression. found in the catalog.
Pollen-specific gene expression.
Scott James Tebbutt
Thesis (Ph.D.),University of East Anglia, School of Biological Sciences, 1993.
by studying pollen-specific gene expression. Male gametogenesis in flowering plants, from archesporal cells to mature pollen, involves a series of complicated events (Goldberg et al., ). A wide variety of specific gene products are involved. The differentiation and development of the male gameto-phyte of angiosperms depend on the expression ofCited by: GENE EXPRESSION deals with gene structure, gene organization, genome evolution, gene function, transcription, RNA processing, genetic code, translation, and fate of finished proteins, ribosomes, and used messenger RNAs. At the end, an overview of gene expression has been provided. This book provides comprehensive knowledge on these aspects of. A pollen-specific cDNA clone (SF17) has been isolated from a sunflower floral cDNA cDNA encodes a leucine-rich protein of amino acid residues with a molecular mass of kDa. Structural features of the predicted polypeptide include an N-terminal hydrophyllic domain, a region containing blocks of leucine-rich repeat elements (LRR) and two hydrophobic Cited by: 7. Plant Gene Expression Center, United States Department of Agriculture-Agricultural Research Service and University of California at Berkeley, Buchanan Street, Albany, California Promoter sequences that direct pollen-specific expression have been previously identified in the LAT59 (for late anther tomato) gene.
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At the applied level, the studies on the pollen and male sporophyte-specific gene expression, and of promoters and transcription factors of relevant genes have the potential to manipulate the fertility in certain cash crops leading to agricultural : Springer US. Pollen-specific gene expression in transgenic plants: coordinate regulation of two different tomato gene promoters during microsporogenesis DAVID TWELL, JUDY YAMAGUCHI and SHEILA McCORMICKCited by: Fabijanski, SF A gene showing sequence similarity to pectin esterase is specifically expressed in developing pollen of Brassica napus.
Sequences in its 5’ flanking region are conserved in other pollen-specific by: 1. Pollen-specific gene expression One interesting result of the recent reports [ 6 – 9 ] is the identification of pollen-specific genes, which may be involved directly in aspects of pollen biology, such as in pollen-tube growth and guidance or in by: From the Back Cover.
This book offers a comprehensive look into the science of gene expression and regulation. Focusing on topics such as actions of nuclear receptors, RNA processing, and DNA methylation and imprinting, Gene Expression and Regulation is edited by a leading biologist and includes contributions by experts in the by: Summary To investigate the regulation of gene expression during male gametophyte development, we analyzed the promoter activity of two different genes (LAT52 and LAT59) from tomato, isolated on the basis of their anther-specific expression.
Pollen-specific gene expression One interesting result of the recent reports [ 6 - 9 ] is the identification of pollen-specific genes, which may be involved directly in aspects of pollen biology, such as in pollen-tube growth and guidance or in by: ZmSTK2_USP, located on the long arm of chromosome 4, belongs to the serine/threonine kinase gene in sequence analysis of bp upstream from the start codon ATG has shown that it contains cis-element motifs and two types of anther/pollen-specific promoter elements (GTGA and AGAAA), suggesting that it is the pollen-specific Cited by: 5.
quences that could not alone confer high-leve1 pollen-specific gene expression. Thus, although all of these experiments de- fined promoter sequences important for expression in pollen, they did not delimit pollen specificity elements in the promoter proximal regions.
Functional analysis of other pollen-expressedCited by: The regulation of pollen-specific gene expression is based on Pollen-specific gene expression. book interactions between transcription factors and cis-acting regulatory elements in the promoters of pollen specific genes.
In this situation, we thought the expression of this reference gene (β-actin) was generally constant in the tested samples. Reviewer 2 Report. Authors have demonstrated that the p promoter, 29 a short and high-activity promoter can drive pollen-specific expression of transgenes in rice and Arabidopsis transformation experiments.
SlCRK1 has pollen-specific cis -acting elements in the promoter region, and its promoter has pollen-specific activity in the homozygous transgenic plants of tomato and Arabidopsis as confirmed through histochemical GUS assays.
Moreover, the expression of SlCRK1 is not detected via stress treatment or hormone by: 2. A third gene, ZmABP3, encodes a protein only 56% and 58% identical to ZmABP1 and ZmABP2, respectively, and its expression is suppressed in pollen and germinated pollen. promoter of the LAT59 gene that are necessary and suffi- cient to drive pollen-specific gene expression (Eyal et ai., ).
The constructs used for those experiments did not contain the sequence encoding the 5' UTR region of LAT59 because we had found that it negatively affected gene ex- pression.
To further characterize this region of the LAT59Cited by: Twell D., Yamaguchi J., McCormick S. () Pollen specific gene expression in transgenic plants: coordinate regulation of two different tomato gene promoters during microsporogenesis. development – PubMed Google ScholarCited by: 3.
Pollen Grains Accumulate a Restricted and Unique Set of Genes. We used Arabidopsis GeneChip ATH1 oligonucleotide arrays, represent annotated genes of the Arabidopsis genome, to establish the gene expression profile of fluorescence-activated cell sorted, hydrated pollen grains (Becker et al., ) in comparison to vegetative tissues (all.
Baltz, R., C. Domon, D.T.N. Pillay, and A. Steinmetz. Isolation and characterization of a pollen-specific gene from sunflower encoding a zinc finger by: 2.
Earlier, a pollen-specific Oryza sativa indica pollen allergen gene (OSIPA), coding for expansins/pollen allergens, was isolated from rice, and its promoter--upon expression. Pollen-specific gene expression in transgenic plants: Coordinate regulation of two different tomato gene promotors during microsporogenesis Article (PDF Available) in Development (3) Pollen-specific gene expression in transgenic plants: coordinate regulation of two different tomato gene promoters during microsporogenesis.
Twell D(1), Yamaguchi J, McCormick by: By analysing transgenic plants containing chimaeric genes consisting of chiA and chiB promoter fragments and the GUS reporter gene, a kb chiA P A2 promoter fragment that drives pollen-specific gene expression and a kb chiB P B promoter fragment that confers anther-specific (pollen and tapetum cells) expression to the GUS gene were Cited by: Conclusions We demonstrate that constitutive expression in leaves of the pollen-specific gene SKS13 can enhance plant defence, resulting in a reduction of M.
persicae population development and. In this article we will discuss about the pollen-expressed and pollen specific genes. It is recognized that there are two different sets of genes required for pollen development, one set for the development of pollen grains prior to anthesis.
Pollen Gene Expression: Molecular Evidence Joseph P. Mascarenhas Department of Biological Sciences, State University of New York at Albany Albany, New York I. Introduction There has been a dramatic increase in the number of laboratories studying genes that are expressed in the male gametophyte and in the isolation and analysis of pollen-specific by: Pollen-specific expression of AtPPME1.
To examine potential tissue specificity of the At1g gene expression, we utilized the Fluorescent Tagging of Full-Length Proteins (FTFLP) technique (Tian et al., ).In this approach, which has been shown to faithfully reproduce the native level and pattern of gene expression as well as subcellular localization of the fluorescently tagged gene Cited by: Promoter sequences that direct pollen-specific expression have been previously identified in the LAT59 (for late anther tomato) gene.
Here, we show that the LAT59 sequences encoding the 5' untranslated region inhibit expression of reporter genes by > fold in transient expression experiments and up to fold after stable by: This pollen-specific expression was further confirmed by microarray data from the AtGenExpress project, in which AtAMT1;4 was reported to be specifically expressed in the tissue of stamens (ATGE_36) or mature pollen (ATGE_73), but not in other floral parts, such as sepals, petals, carpels and pedicels, or in any other Arabidopsis tissue (Schmid et al.
).Cited by: The present invention relates to regulatory elements for tissue specific, in particular pollen specific, expression of genes of interest in plants.
The invention also relates to methods to obtain transgenic plants which show pollen specific expression of genes of interest and to methods for isolating pollen specific genes and/or pollen specific regulatory by: 3.
Duplications allow for gene functional diversification and accelerate genome evolution. Occasionally, the transposon amplification machinery reverse transcribes the mRNA of a gene, integrates it into the genome, and forms an RNA-duplicated copy: the retrogene.
Although retrogenes have been found in plants, their biology and evolution are poorly understood. Here, Cited by: Regulation of Gene Expression: Molecular Mechanisms is intended to serve as either a textbook for graduate students or as a basic reference for laboratory personnel.
Indeed, we are using this book to teach a graduate-level class at The Pennsylvania State : Gary H. Perdew, Jack P. Vanden Heuvel, Jeffrey M.
Peters. PSG is a pollen-specific gene isolated from wheat. The kb promoter upstream of the ATG start codon was isolated by inverse-PCR (IPCR). To determine its activity, the PSG promoter was fused with the β-glucuronidase (GUS) reporter gene and introduced into tobacco.
Histochemical analysis in transgenic tobacco showed that GUS activity was Cited by: Pollen-specific expression from PSI-GUS was also observed when the chimeric gene was introduced into wild type tobacco tissues in these transient expression assays (Fig. 9D). The PSI -GUS chimeric gene was unable to express P-glucuronidase activities in leaf tissues from rice and tobacco (Fig.
9C, E). All three pollen Rops fall into the same subgroup as Rop1Ps and diverge from those Rops that are not expressed in mature pollen, suggesting a coupling of the structural conservation of Rop GTPases to their gene expression in pollen. However, pollen-specific transcript accumulation for Rop1At is much higher than that for Rop3At and Rop5At.
We demonstrate that constitutive expression in leaves of the pollen-specific gene SKS13 can enhance plant defence, resulting in a reduction of M. persicae population development and also decreases the transmission of persistent by: 3.
Plants have developed a variety of mechanisms to counteract aphid attacks. They activate their defences by changing the expression of specific genes. Previously we identified an activation tag mutant of Arabidopsis thaliana on which Myzus persicae population development was reduced.
Activation tag mutants are gain-of-function in which the expression of a gene is Cited by: 3. for pollen-specific gene expression are discussed. INTRODUCTION Little is known of how gene expression changes during the alternating diploid and haploid generations of vascular plants.
Studies indicate that most plant genes are expressed in both the diploid stage, or sporophyte, and the haploid stage, or gametophyte (Ottaviano and Mulcahy, ).Cited by: Abstract; PDF ( K) PDF-Plus ( K) Figures; Citing articles; SlPMEI, a pollen-specific gene in tomato. Woong Bom Kim, 1 2 5 Chan Ju Lim, 1 5 Hyun A.
Jang, 1 2 So Young Yi, 1 Sang-Keun Oh, 4 Ha Yeon Lee, 1 Hyun A. Kim, 1 3 Youn-Il Park, 2 Suk-Yoon Kwon 1 3 1 Green Bio Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon Cited by: 3.
We have characterized the global gene expression patterns of Arabidopsis pollen using Serial Analysis of Gene Expression (SAGE).
A total of 21, SAGE tags were sequenced and 4, unique tags were identified. Interestingly, the number of unique tags in pollen was low compared with the SAGE library of the leaf constructed on a similar by: We also used the promoter of the MGH3 gene, a histone H3 variant gene with well-characterised generative and sperm cell-specific expression at high levels.
The ability of these pollen-specific promoters to permit generation of mature artificial microRNAs is shown in Cited by: ISBN: OCLC Number: Description: xv, pages: illustrations ; 24 cm: Contents: Pt. Gene Expression.
Molecular Biology of. Other important topics covered in the volume include newly discovered effects of fatty acids on regulating gene expression, the effects of diet on mRNA editing, the interplay between dietary carbohydrates and proteins in regulating metabolism of liver cells, the effects of metal ions on protein synthesis, and much by: 5.Identification and in Situ Localization of Pollen-Specific Genes S.
P. Davies, M. B. Singh, and R. B. Knox School of Botany, University of Melbourne Parkville, VictoriaAustralia I. Introduction By isolating genes that are expressed exclusively during pollen development, a detailed analysis of cell-specific gene regulation and cellular differentiation has become a by: Pollen biotechnology: gene expression and allergen characterization.
and M.B. Singh; Anther- and pollen-specific gene expression in sunflower-- Andre Steinmetz, Rachel Baltz, Claire Domon and Jean-Luc Evrard; Gene expression in developing maize pollen-- D.A. Hamilton; In vitro pollen cultures: progress and perspectives-- E.